EPA Method 8328 is a cutting-edge analytical procedure designed for the accurate detection and quantification of per- and polyfluoroalkyl substances (PFAS) in various environmental samples. This method is pivotal in addressing the growing concerns about PFAS contamination in both residential and natural environments.
Discover EPA Method 8328: Your essential guide to advanced PFAS testing in environmental samples, tailored for consultants and homeowners.
In this article, we cover the comprehensive aspects of EPA Method 8328, detailing its significance, procedure, and impact for environmental consultants and homeowners.
Related articles:
EPA Method 8327
ASTM 7979
Overview of EPA Method 8328
EPA Method 8328 is a sophisticated analytical technique specifically developed for the detection and quantification of per- and polyfluoroalkyl substances (PFAS) in environmental matrices. It represents a significant advancement in the field of environmental analysis, offering enhanced sensitivity and specificity for PFAS detection.
This method was developed in response to the increasing need for more accurate and reliable PFAS analysis, particularly in light of the growing awareness of PFAS-related environmental and health concerns. It incorporates the latest advancements in analytical chemistry to provide more precise and comprehensive data.
EPA Method 8328 is widely applied in various fields, including environmental science, public health, and industry. In environmental science, it is crucial for monitoring PFAS contamination in water bodies, soil, and sediments, aiding in the assessment of environmental health and the formulation of remediation strategies.
Public health authorities utilize this method to evaluate the safety of drinking water and to conduct epidemiological studies on the effects of PFAS exposure. In the industrial sector, EPA Method 8328 is instrumental in ensuring that manufacturing processes and waste discharges comply with environmental regulations regarding PFAS.
Its ability to accurately detect a wide range of PFAS compounds makes it an invaluable tool for addressing the complex challenges posed by these persistent environmental pollutants.
Read my article on Analytical methods for PFAS for additional details.
Analytes for EPA Method 8328
The following table includes the list of analytes for EPA Method 8328, their acronym, and CAS number.
CAS#:
| Analyte | Acronym | CAS# |
| PFAS Sulfonic Acids | ||
| Perfluoro-1-butanesulfonic acid | PFBS | 375-73-5 |
| Perfluoro-1-pentanesulfonic acid | PFPeS | 2706-91-4 |
| Perfluoro-1-hexanesulfonic acid | PFHxS | 355-46-4 |
| Perfluoro-1-heptanesulfonic acid | PFHpS | 375-92-8 |
| Perfluoro-1-octanesulfonic acid | PFOS | 1763-23-1 |
| Perfluoro-1-nonanesulfonic acid | PFNS | 68259-12-1 |
| Perfluoro-1-decanesulfonic acid | PFDS | 335-77-3 |
| 1H, 1H, 2H, 2H-perfluorohexane sulfonic acid | 4:2 FTS | 757124-72-4 |
| 1H, 1H, 2H, 2H-perfluorooctane sulfonic acid | 6:2 FTS | 27619-97-2 |
| 1H, 1H, 2H, 2H-perfluorodecane sulfonic acid | 8:2 FTS | 39108-34-4 |
| PFAS Carboxylic Acids | ||
| Perfluorobutanoic acid | PFBA | 375-22-4 |
| Perfluoropentanoic acid | PFPeA | 2706-90-3 |
| Perfluorohexanoic acid | PFHxA | 307-24-4 |
| Perfluoroheptanoic acid | PFHpA | 375-85-9 |
| Perfluorooctanoic acid | PFOA | 335-67-1 |
| Perfluorononanoic acid | PFNA | 375-95-1 |
| Perfluorodecanoic acid | PFDA | 335-76-2 |
| Perfluoroundecanoic acid | PFUnDA | 2058-94-8 |
| Perfluorododecanoic acid | PFDoDA | 307-55-1 |
| Perfluorotridecanoic acid | PFTrDA | 72629-94-8 |
| Perfluorotetradecanoic acid | PFTeDA | 376-06-7 |
| PFAS Sulfonamides and Sulfonamidoacetic Acids | ||
| N-ethylperfluoro-1-octanesulfonamidoacetic acid | N-EtFOSAA | 2991-50-6 |
| N-methylperfluoro-1-octanesulfonamidoacetic acid | N-MeFOSAA | 2355-31-9 |
| Perfluoro-1-octanesulfonamide | PFOSA | 754-91-6 |
Procedure and Process
EPA Method 8328 employs a meticulous procedure that begins with the collection of environmental samples, followed by their preparation using specific extraction techniques. The prepared samples are then analyzed using advanced liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS).
This process involves the separation of PFAS compounds on a chromatographic column, followed by their detection and quantification using the mass spectrometer. The equipment used, including high-performance LC systems and sensitive mass spectrometers, is pivotal for the method’s high precision. EPA Method 8328 stands out for its ability to accurately identify and quantify a wide array of PFAS compounds simultaneously, leveraging the principles of chromatographic separation and mass spectrometric detection.
Its accuracy and sensitivity are further enhanced by the use of isotopically labeled surrogates and internal standards, ensuring reliable quantification even in complex matrices.
Accuracy and Sensitivity
EPA Method 8328 is highly acclaimed for its exceptional accuracy and sensitivity in detecting PFAS compounds. The method’s sensitivity is showcased by its low detection limits, essential for identifying trace levels of PFAS in environmental samples.
This accuracy is achieved through meticulous sample preparation, precise chromatographic separation, and the use of advanced mass spectrometry techniques. The method’s reliability is further supported by stringent quality control measures, including the use of blanks, duplicates, and matrix spikes.
This ensures that EPA Method 8328 provides dependable results, making it a preferred choice for environmental monitoring and public health assessments where accurate detection of low-level PFAS is crucial.
Comparison with Other Methods
EPA Method 8328 offers unique advantages and some limitations when compared to other PFAS testing methods. Below is a table comparing EPA Method 8328 with other prevalent PFAS analysis techniques, highlighting its strengths and potential drawbacks.
| Method | Sensitivity | Range of PFAS Detected | Sample Matrix Compatibility | Equipment Complexity |
| EPA Method 8328 | High | Broad spectrum of PFAS compounds | Versatile (water, soil, wastewater) | Advanced (LC/MS/MS) |
| EPA Method 537.1 | Moderate | 18 PFAS compounds, primarily perfluoroalkyl carboxylic acids and sulfonic acids | Mainly drinking water | Moderate (LC/MS/MS) |
| ASTM D7979 with Method D7968 | High | Extensive range, including newer and less common PFAS | Environmental waters, including non-potable | Advanced (LC/MS/MS) |
| ISO 25101 | Moderate to High | Limited to perfluoroalkyl sulfonic acids, carboxylic acids, and sulfonamidoethanols | Water, including drinking and surface water | Moderate to Advanced (LC/MS/MS) |
For an overview of Draft Method 8328 and its applications in various matrices, visit the American Water Works Association’s resource.
Practical Implications of the Method
For Environmental Consultants
For environmental consultants, EPA Method 8328 is an indispensable tool, particularly in conducting thorough site assessments, ensuring regulatory compliance, and guiding remediation projects. This method allows for the precise detection and quantification of a wide range of PFAS compounds in various environmental matrices, which is crucial for evaluating the extent of contamination at a site. It plays a pivotal role in the development of remediation strategies, as accurate data on PFAS levels are essential for determining the appropriate course of action. Additionally, EPA Method 8328 aids consultants in advising their clients on compliance with environmental regulations, as it aligns with the stringent standards set by environmental protection agencies for PFAS analysis.
Learn more about the methodology and scope of PFAS analysis in EPA Method 8327 at the EPA’s official page.
For Homeowners
For homeowners, EPA Method 8328 is particularly relevant in the context of home water testing and property evaluations. This method provides a means to ensure that their drinking water and property are free from harmful levels of PFAS, a growing concern in many communities.
Homeowners in areas known for industrial pollution or near military bases, where PFAS compounds are commonly used, can rely on laboratories that use EPA Method 8328 for accurate testing. This method offers peace of mind by providing reliable data on the presence of PFAS in residential water sources and properties.
Cost and Accessibility
The cost and accessibility of EPA Method 8328 can vary, depending on several factors such as the complexity of the sample matrix and the level of sensitivity required. For environmental consultants, the investment in this method is often justified by the need for high accuracy and compliance with regulatory standards.
However, for homeowners, the cost might be a consideration, as testing for PFAS using this method can be more expensive than standard water tests. Accessibility is primarily through professional laboratories equipped with the necessary LC/MS/MS systems. One potential challenge for both consultants and homeowners is finding a local laboratory with the capability and certification to perform EPA Method 8328, as it requires specialized equipment and trained personnel.
For detailed insights into December 2019’s Draft Method 8328 for PFAS, check out the information available at Environmental Standards Inc..
Conclusion
EPA Method 8328 stands as a pivotal advancement in the detection and analysis of PFAS, offering unparalleled accuracy and sensitivity. Its application is crucial for environmental consultants in professional assessments and remediation, as well as for homeowners concerned about water and property safety.
Despite its higher cost and need for specialized equipment, the method’s reliability and compliance with stringent regulatory standards make it an invaluable tool in the ongoing efforts to address PFAS contamination.